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Project properties |
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| Title | Understanding the role of gene duplications in cereals using CRISPR |
| Group | Molecular Biology, Laboratory of |
| Project type | thesis |
| Credits | 12-39 |
| Supervisor(s) | Wilma van Esse |
| Examiner(s) | Wilma van Esse |
| Contact info | wilma.vanesse@wur.nl |
| Begin date | 2024/10/01 |
| End date | 2028/10/01 |
| Description | Targeted mutagenesis is a important part in our research to unravel the role of key regulatory genes in plant development. Within this project, we will CRISPR-Cas in barley on key developmental genes to understand their role in shaping plant architecture and development in this crop.
Typical student projects involve construct design, using Golden Gate cloning, for targeted mutagenesis. Depending on the length of the thesis (BSc/MSc) and project availability, you will also gain experience with plant transformation to perform genome editing and analyse the mutations/deletions created. |
| Used skills | one or more of the technologies below:
• Protein-protein or protein-DNA interaction studies • Phylogenetic analysis • Conserved domain analysis using natural allelic variation • Selection CRISPR-CAS9 mutagenized plants in barley • Project management, writing and presenting your thesis |
| Requirements | For BSc thesis: MOB20306 or GEN20806
For MSc thesis: MOB-20306 and MOB-30306 or MOB-30806 or MOB-31303 or PHP-30806 |