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Project properties |
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| Title | Characterization of changes in plasma membrane proteome |
| Group | Cell Biology |
| Project type | thesis |
| Credits | 12 onwards |
| Supervisor(s) | Peter Grones |
| Examiner(s) | Peter Grones |
| Contact info | peter.grones@wur.nl |
| Begin date | 2024/02/01 |
| End date | 2026/01/01 |
| Description | Plant plasma membrane undergoes constant recycling of its components, by degrading the old proteins and delivering the fresh one. However, our knowledge of how the PM signaling proteins respond to different environmental stimuli is very limited. Moreover, not all PM proteins need to be degraded after the stress stimuli, as their function can be altered by post-translational modifications (PTM) (e.g. phosphorylation). There are several open questions in this topic: Which proteins are changing their localization during cellular response to external cues? How is the selection regulated? What is the role of PTMs in this process? |
| Used skills | Advanced confocal microscopy, molecular cloning, working with mutant lines, cell culturing, PCR and DNA cloning |
| Requirements | recommended; CLB-30306 |