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Project properties |
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| Title | Role of Ubiquitin Proteasome System in Gamete Interaction. |
| Group | Molecular Biology, Laboratory of |
| Project type | thesis |
| Credits | 21-36 |
| Supervisor(s) | Dawit Tekleyohans |
| Examiner(s) | Prof. Dr. Gerco Angenent |
| Contact info | dawit.tekleyohans@wur.nl |
| Begin date | 2024/01/01 |
| End date | 2028/01/01 |
| Description | Protein ubiquitination is one mode of post-translational modification mechanism essential in several biological processes. It involves covalent attachments of ubiquitin to the lysine residue of a substrate through ubiquitin proteasome system (UPS) that is comprised of three key enzymes (E1, E2, and E3). The E3 ligase serves as a meeting platform between the target substrate and the ubiquitin. In plants, there are several E3 ligases but only few are functionally characterized. In this project, we aim to identify those E3 ligases that are specifically expressed in the male and female gametes and investigate their role in plant reproduction using several state of the art techniques. The outcome of this research will enable us to understand further about plant reproduction and open opportunities to improve breeding activities. |
| Used skills | Genome editing via CRISPR/Cas9, Imaging via confocal laser scanning microscopy (CLSM), Expression analysis using RTq-PCR, gene cloning and generation of transgenic plants, protein-protein interaction assays using yeast-two hybrid method |
| Requirements | For BSc thesis: MOB20306 or GEN20806 or equivalent
For MSc thesis: MOB20306 and MOB30306 or MOB31303 or MOB30806 or PHP30806 or equivalent |