Project properties

Title Determination of Fungal Biomass using VNIR/MIR spectroscopy
Group Soil Geography and Landscape
Project type thesis
Credits 36
Supervisor(s) Titia Mulder (SGL), Rachel Creamer (SBL), Fenny van Egmond (WENR)
Examiner(s) Titia Mulder
Contact info titia.mulder@wur.nl
Begin date 2022/01/03
End date
Description VNIR and MIR spectroscopy for analysing physicochemical soil properties has proven to be efficient and relatively accurate compared to wet chemistry methods. Recently, studies have started exploring the use of VNIR/MIR spectroscopy for estimating microbial biomass concentration, based on the amount of Phospholipid Fatty Acids (PLFAs). PLFA profiles reflect the fingerprint of microbial communities and classes of PLFA are used as biomarkers for different taxonomic or functional groups of soil microorganisms. In this project you will explore if and how VNIR and MIR spectroscopy can be used to estimate fungal biomass. Laboratory analysis will be part of the thesis work. A comprehensive spectral feature analysis will be needed to provide insight in key absorption features of fungi. Moreover, sophisticated statistical modelling using spectral libraries will be used for quantifying the fungal biomass.

Further reading: https://doi.org/10.1016/j.soilbio.2021.108319
Used skills Laboratory analysis, R scripting
Requirements Understanding of Soil Biology and Soil Spectroscopy