Project properties

Title Insights on arbuscular mycorrhizal fungi activity in soil by measuring the length of fungal hyphae
Group Soil Biology
Project type thesis
Credits 24-39
Supervisor(s) Alix Vidal, Laura Ferron
Examiner(s) prof.dr. Gerlinde De Deyn
Contact info alix.vidal@wur.nl
Begin date 2022/02/01
End date 2022/07/31
Description Arbuscular mycorrhizal fungi (AMF) are crucial to provide phosphorus (P) to plants in limiting P conditions. One way of measuring AMF activity is to look at colonization rate in plant roots. However this technic is only providing a snapshot of the relationship between plants and AMF. Measuring the length of fungal hyphae should give more insight into the intensity of the activity. During this thesis, you will set up this methodology in the Soil Biology Lab from a provided protocol and use it on an existing set of samples from a field experiment testing a combination of novel P fertilizers and earthworm diversity to promote plant P uptake. You will compare these results to the well-established root colonization method. Finally, you will be able to put this data into perspective using the existing data of this field experiment.
Aims:
- Set up a methodology to measure the length of fungal hyphae and test it on a set of existing samples.
- Measure the root colonization by AMF
- Put the results into perspective using root data and an existing dataset.
Used skills - Laboratory work to set up and use the new method
- Help to draft the standard operating protocol of the Soil Biology Lab for this method
Requirements Handbook for the generic thesis requirements at SBL, https://ssc.wur.nl/Handbook/Course/SBL-81836