Project properties

Title Optimization transformation protocols for cabbage (Brassica oleracea)
Group Plant Breeding, Laboratory of
Project type thesis
Credits 24-39
Supervisor(s) Dr. Guusje Bonnema, Zihan Liu
Examiner(s) Dr. Guusje Bonnema
Contact info Guusje Bonnema, Guusje.Bonnema@wur.nl, tel. 0317-484028
Begin date 2021/03/01
End date 2022/08/01
Description Brassica species display enormous phenotypic variation: B. oleracea for example includes cauliflowers, cabbages, kales and kohlrabi. Brassica genomes all have undergone a whole genome triplication (WGT) which facilitated this phenotype diversification. We study the so called domestication events. Previous research resulted in several candidate genes for leafy head formation. We like to perform functional analyses with these candidate genes to confirm their roles in leafy head formation. One approach is to generate genotypes overexpressing these genes, or knocking these genes out using CRISPR CAS. For both strategies we need a good transformation protocol. The main aim of the proposed thesis is to develop a transformation protocol for Brassica oleracea, with special focus on the cabbages.
Used skills Plant Cell and Tissue Culture experiment; molecular skills to analyse putative transformants. Possible gene cloning skills (most constructs are already available).
Requirements Plant Cell and Tissue Culture PPH30306;