Project properties |
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Title | Optimization transformation protocols for cabbage (Brassica oleracea) |
Group | Plant Breeding, Laboratory of |
Project type | thesis |
Credits | 24-39 |
Supervisor(s) | Dr. Guusje Bonnema, Zihan Liu |
Examiner(s) | Dr. Guusje Bonnema |
Contact info | Guusje Bonnema, Guusje.Bonnema@wur.nl, tel. 0317-484028 |
Begin date | 2021/03/01 |
End date | 2022/08/01 |
Description | Brassica species display enormous phenotypic variation: B. oleracea for example includes cauliflowers, cabbages, kales and kohlrabi. Brassica genomes all have undergone a whole genome triplication (WGT) which facilitated this phenotype diversification. We study the so called domestication events. Previous research resulted in several candidate genes for leafy head formation. We like to perform functional analyses with these candidate genes to confirm their roles in leafy head formation. One approach is to generate genotypes overexpressing these genes, or knocking these genes out using CRISPR CAS. For both strategies we need a good transformation protocol. The main aim of the proposed thesis is to develop a transformation protocol for Brassica oleracea, with special focus on the cabbages. |
Used skills | Plant Cell and Tissue Culture experiment; molecular skills to analyse putative transformants. Possible gene cloning skills (most constructs are already available). |
Requirements | Plant Cell and Tissue Culture PPH30306; |