Project properties

Title De-evolution of the wing: how doublesex determines wing size in Nasonia vitripennis
Group Entomology, Laboratory of
Project type thesis
Credits 36
Supervisor(s) F. Guerra (ENT), E. Verhulst (ENT), B. Pannebakker (GEN)
Examiner(s) Marcel Dicke (ENT)
Contact info filippo.guerra@wur.nl
https://wageningenur4-my.sharepoint.com/:f:/g/personal/filippo_guerra_wur_nl/En7sBa8E5cFBkgiEyyaN3zwB3Dcb_MQJZb-b_k8RniEQPw?e=nNL0qW
Begin date 2020/01/31
End date 2020/01/31
Description In the most studied parasitoid wasp, Nasonia vitripennis, males have tiny wings and cannot fly, whereas females have long wings and do fly. This difference is due to the amount of sex-specific isoforms of Doublesex, a transcription factor responsible for sex specific expression of many genes and ultimately sexual differentiation. Yet, we don�t know which of the many genes targeted by Doublesex cause sex specific wing development in Nasonia. So, in this project you will identify some of these genes and ow they are regulated by DSX . This study is crucial to determine the pathways involved in wing formation and the way that evolution affects (and is affected by) them.
In this project you will:
- find candidate genes based on their ontology and expression pattern;
- design the dsRNA and perform RNAi knock-downs via microinjections;
- use qPCR to determine knock-down efficiency;
- phenotype RNAi treated wasps
The project is based in the Laboratory of Entomology and the Laboratory of Genetics, and needs a student with a strong background in molecular biology.
Used skills RNAi, qPCR, insect rearing, guided analysis of transcriptomic data
Requirements For doing a BSc-thesis at Entomology, there are no requirements for specific courses.
For doing a MSc-thesis or internship at Entomology, the following requirements apply: ENT-30806 + a second ENT-course (preferably ENT-30306 or ENT-50806 or ENT-53806). As an alternative for the second ENT-course, PHP-30806 or BHE-30306 can be selected.
Note: these requirements do not apply for MBI students; MBI students should check the requirements for doing an ENT MSc-thesis or internship in the study programme of their specialisation.