|
Project properties |
|
| Title | Dissecting Xanthomonas black rot resistance in cabbage |
| Group | Biosystematics Group |
| Project type | thesis |
| Credits | 24-36 |
| Supervisor(s) | Dr. Klaas Bouwmeester |
| Examiner(s) | Prof. dr. Eric Schranz |
| Contact info | klaas.bouwmeester@wur.nl |
| Begin date | 2019/11/29 |
| End date | 2024/07/31 |
| Description | Black rot, caused by the bacterium Xanthomonas campestris campestris (Xcc), is one of the most destructive diseases of cabbage. Currently grown cultivars do not provide adequate resistance, and no resistance genes have been cloned so far.
This project aims to unravel novel defense mechanisms during early bacterial attack. You will: - explore natural variation in black rot resistance using bio-panels of related cabbage species (Brassica rapa, B. oleracea and B. napus) and diverse wild relatives. - quantify expression of candidate genes in differential Xcc-Brassica interactions. - characterize candidate genes for resistance against black rot. - set-up new methods for gene-silencing in Brassica, and test mutants with diverse Xcc isolates to detect changes in disease susceptibility. |
| Used skills | Molecular biology techniques (DNA/RNA isolation, cloning, qPCR),
Plant phenotyping, Disease assays with plant pathogens |
| Requirements | Basic knowledge of plant-microbe interactions and molecular biology |