Project properties

Title Genetic mapping of a novel potato late blight resistance gene
Group Plant Breeding, Laboratory of
Project type thesis
Credits 18-39
Supervisor(s) Jack Vossen, Gert van Arkel
Examiner(s) Ronald Hutten, Jack Vossen
Contact info jack.vossen@wur.nl
Begin date 2017/04/01
End date 2018/07/01
Description Late blight, caused by Phytophthora infestans, is the most devastating disease for potato crops. Sustainable and durable resistance strategies are currently not available however stacking of multiple resistance genes (R genes) is a promising approach for disease management. Our group aims to identify and clone sufficient resistance genes for a sustainable Late Blight resistance strategy through R gene stacking.
Genes from wild potato relatives that cause resistance to late blight can be mapped and cloned with the aid of molecular markers.
In this thesis project, you will use a population that is segregating for a novel resistance gene (Rpi-cap1) from Solanum capsicibacatum. You will design a strategy to identify single nucleotide polymorphisms (SNPs) that are linked to the resistance gene. The SNPs will be used to develop molecular markers for genetic mapping. This will involve whole genome sequencing of the resistant and susceptible parents of the population. Also bulks of resistant and susceptible individuals which will allow you to pursue a bulked segregant analysis approach (BSA).
Used skills - Late blight resistance assays
- Agroinfiltration
- SNP mining in whole genome sequencing data
- Genomic DNA isolation
- Bulked segregant analysis
- Genetic mapping
Requirements