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Project properties |
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| Title | Unravelling the role of PLT transcription factors during early embryogenesis |
| Group | Molecular Biology, Laboratory of |
| Project type | thesis |
| Credits | 18-39 |
| Supervisor(s) | Viola Willemsen |
| Examiner(s) | Prof dr B Scheres (PDB/MOB) |
| Contact info | Viola.Willemsen@wur.nl |
| Begin date | 2014/12/01 |
| End date | 2019/12/01 |
| Description | Embryogenesis is a crucial developmental process in flowering plants. The initial asymmetric division of the plant zygote produces different apical and basal daughter cells and has been linked with differential auxin distribution but the underlying mechanism for this generation of cell fate diversity remains unknown. Expression of the PLETHORA (PLT) genes, encoding AP2 domain containing stem cell transcription factors, is detectable from the earliest stages of embryogenesis onward. Two members of these plant specific transcription factor family are crucial at the first stages of embryogenesis. The function of PLT genes in early embryogenesis will be investigated. And we will try to isolate and analyse factors which are activating these important transcription factors. |
| Used skills | Techniques your are going to use in this project are:
� Standard molecular biological tools: (gate way)cloning, PCR, DNA isolation, sequencing, gel electrophorese, protein expression in bacteria, protein isolation, PAGE � Yeast-one-hybrid � Yeast-two-hybrid � EMSA � Confocal scanning microscopy � In Situ Hybridisation � Mutant analysis � Bioinformatics |
| Requirements | For BSc thesis: MOB20306
For MSc thesis: MOB20306 and MOB30306 or MOB31303 or MOB30806 or PHP30806 or equivalent |