Project properties

Title Functional analysis of flavour-related genes of tomato
Group Plant Breeding, Laboratory of
Project type thesis
Credits 30-39
Supervisor(s) Dr. Arnaud Bovy (arnaud.bovy@wur.nl), Dr. Sjaak van Heusden (sjaak.vanheusden@wur.nl)
Examiner(s) Richard Visser
Contact info Dr. Arnaud Bovy (arnaud.bovy@wur.nl), Dr. Sjaak van Heusden (sjaak.vanheusden@wur.nl)
Begin date 2012/02/18
End date 2024/01/01
Description Taste is an important trait in tomato breeding. Due to its complex multifactorial nature, breeding for improved taste has always been difficult. In the past five years we have been participating in a large research program aimed at unravelling the taste of tomatoes (www.cbsg.nl). A large association study allowed the identification of several metabolites important for the taste of tomato. Currently, our efforts are focused on the isolation of the genes involved in the production of these metabolites. We recently found that many taste-related metabolites are stored as glycosides and that glycosylation plays an important role in the regulation of taste-related metabolic pathways. Several candidate glycosyltransferase genes have been isolated
The current project aims to elucidate the function of candidate glycosyltransferase genes through transient expression or VIGS-induced silencing in tomato fruits. This involves subcloning of candidate genes into appropriate expression vectors, transient transformation of tomato fruits and biochemical analysis of transformed fruits to determine the effect of the introduced gene.
Used skills
Requirements ABG-30306 Genomics,
MIB-21306 Bioinformatics,
GEN-30306 Genetic Analysis, Tools and Concepts (GATC),
BPE-30303 Metabolic Modelling and Pathway Analysis