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Project properties |
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| Title | Regeneration recalcitrance in plants |
| Group | Molecular Biology, Laboratory of |
| Project type | thesis |
| Credits | 18-39 |
| Supervisor(s) | Janna Wittmer |
| Examiner(s) | Dr. R Heidstra (PDB/MOB) |
| Contact info | janna.wittmer@wur.nl |
| Begin date | 2018/11/01 |
| End date | 2024/10/01 |
| Description | Regeneration recalcitrance is a problematic feature of several crop species, which prevents efficient scientific research in these crops. In this project you will attempt to obtain information on regeneration recalcitrance by using transformation and a unique regeneration method on several different crops. In addition, you will perform tissue culture experiments in Arabidopsis. Together, these data should yield greater insight in the mechanism of recalcitrance. |
| Used skills | Standard recombinant DNA technology such as DNA isolation, PCR and (Golden Gate) cloning.
Handling and transformation of model organisms; E.coli, agrobacterium, Arabidopsis. Gene expression analysis by way of quantitative RT-PCR. Analysis of promoter activity in planta by confocal laser microscopy. Tissue culture. Handling of, and transformation in multiple crop species. Crossing and analysis of these by the above methods. |
| Requirements | For BSc thesis: MOB20306
For MSc thesis: MOB20306 and and MOB30306 or MOB31303 or MOB30806 or PHP30806 or equivalent |